Mito-Lapronic/pGW1
(Plasmid
#140754)
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PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGW1
- Backbone size w/o insert (bp) 5671
- Total vector size (bp) 7748
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMito-Lapronic
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SpeciesSynthetic
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Insert Size (bp)2076
- Promoter CMV
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Tag
/ Fusion Protein
- Mitochondrial Sequence Signal (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SgrAI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mito-Lapronic/pGW1 was a gift from Luis Felipe Barros (Addgene plasmid # 140754 ; http://n2t.net/addgene:140754 ; RRID:Addgene_140754) -
For your References section:
Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded FRET Indicator. Galaz A, Cortes-Molina F, Arce-Molina R, Romero-Gomez I, Mardones GA, Barros LF, San Martin A. Anal Chem. 2020 Jun 29. doi: 10.1021/acs.analchem.0c01741. 10.1021/acs.analchem.0c01741 PubMed 32600029
Map uploaded by the depositor.
