Dead-Lapronic/pcDNA3.1(-)
(Plasmid #140753)

• Purpose: FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio, Negative Control
• Depositing Lab: Luis Felipe Barros
• Publication: Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs.analchem.0c01741.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone size w/o insert (bp): 5422
• Total vector size (bp): 7498
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dead-Lapronic
• Species: Synthetic
• Insert Size (bp): 2076
• Mutation: Changed Histidine 92 to Alanine
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry