pGWB660-CYCD3;1
(Plasmid #140751)

• Purpose: Inducing plant cells to enter mitosis
• Depositing Lab: Bo Liu
• Publication: Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309.

Backbone

• Vector backbone: pGWB660
• Total vector size (bp): 11719
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cyclin D3;1
• Species: A. thaliana (mustard weed)
• Entrez Gene: CYCD3;1 (a.k.a. AT4G34160, CYCD3, CYCLIN D3;1, F28A23.80, F28A23_80)
• Promoter: CaMV 35 promoter
• Tag / Fusion Protein:
  • TagRFP (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: attB1
• 3′ sequencing primer: attB2

Resource Information

• Supplemental Documents:
  • pGWB660-CYCD3;1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry