pGWB660-CYCD3;1
(Plasmid
#140751)
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PurposeInducing plant cells to enter mitosis
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGWB660
- Total vector size (bp) 11719
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCyclin D3;1
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SpeciesA. thaliana (mustard weed)
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Entrez GeneCYCD3;1 (a.k.a. AT4G34160, CYCD3, CYCLIN D3;1, F28A23.80, F28A23_80)
- Promoter CaMV 35 promoter
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Tag
/ Fusion Protein
- TagRFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attB1
- 3′ sequencing primer attB2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB660-CYCD3;1 was a gift from Bo Liu (Addgene plasmid # 140751 ; http://n2t.net/addgene:140751 ; RRID:Addgene_140751) -
For your References section:
Establishment of a mitotic model system by transient expression of the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana benthamiana). Xu J, Lee YJ, Liu B. New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309. 10.1111/nph.16309 PubMed 31679162
