pGWB2-CYCD3;1
(Plasmid
#140750)
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PurposeInducing plant cells to enter mitosis
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB2
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Backbone manufacturer15572
- Total vector size (bp) 16700
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCyclin D3;1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1128
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Entrez GeneCYCD3;1 (a.k.a. AT4G34160, CYCD3, CYCLIN D3;1, F28A23.80, F28A23_80)
- Promoter 35S
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer attB2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGWB2-CYCD3;1 was a gift from Bo Liu (Addgene plasmid # 140750 ; http://n2t.net/addgene:140750 ; RRID:Addgene_140750) -
For your References section:
Establishment of a mitotic model system by transient expression of the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana benthamiana). Xu J, Lee YJ, Liu B. New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309. 10.1111/nph.16309 PubMed 31679162