pLenti CMV TRE3G BCL2L1 Neo
(Plasmid
#140749)
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PurposeInducibly expresses Bcl-xL in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti CMVTRE3G eGFP Neo (w821-1)
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Backbone manufacturer27569
- Backbone size w/o insert (bp) 8804
- Total vector size (bp) 8614
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBCL2L1
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Alt nameBCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52
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SpeciesH. sapiens (human)
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Insert Size (bp)705
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GenBank IDNM_138578.3
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Entrez GeneBCL2L1 (a.k.a. BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52)
- Promoter CMV TRE3G
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV TRE3G BCL2L1 Neo was a gift from Scott Dixon (Addgene plasmid # 140749 ; http://n2t.net/addgene:140749 ; RRID:Addgene_140749) -
For your References section:
Kinetic Heterogeneity of Cancer Cell Fractional Killing. Inde Z, Forcina GC, Denton K, Dixon SJ. Cell Rep. 2020 Jul 7;32(1):107845. doi: 10.1016/j.celrep.2020.107845. 10.1016/j.celrep.2020.107845 PubMed 32640215