pLenti CMV TRE3G BCL2L11 G156E Neo
(Plasmid
#140748)
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PurposeInducibly expresses G156E mutant BIM in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti CMVTRE3G eGFP Neo (w821-1)
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Backbone manufacturer27569
- Backbone size w/o insert (bp) 8804
- Total vector size (bp) 8509
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBCL2L11
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Alt nameBIM
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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MutationChanged Glycine 156 to Glutamate
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GenBank IDNM_138621.5
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Entrez GeneBCL2L11 (a.k.a. BAM, BIM, BOD)
- Promoter CMV TRE3G
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV TRE3G BCL2L11 G156E Neo was a gift from Scott Dixon (Addgene plasmid # 140748 ; http://n2t.net/addgene:140748 ; RRID:Addgene_140748) -
For your References section:
Kinetic Heterogeneity of Cancer Cell Fractional Killing. Inde Z, Forcina GC, Denton K, Dixon SJ. Cell Rep. 2020 Jul 7;32(1):107845. doi: 10.1016/j.celrep.2020.107845. 10.1016/j.celrep.2020.107845 PubMed 32640215