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Addgene

pAKlux1.1
(Plasmid #14074)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14074 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBBR1MCS4
  • Backbone size w/o insert (bp) 4950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bacterial luciferase
  • Alt name
    luxCDABE
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    6954

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKlux1.1 was a gift from Attila Karsi (Addgene plasmid # 14074 ; http://n2t.net/addgene:14074 ; RRID:Addgene_14074)
  • For your References section:

    Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring. Karsi A, Menanteau-Ledouble S, Lawrence ML. FEMS Microbiol Lett. 2006 Jul. 260(2):216-23. 10.1111/j.1574-6968.2006.00310.x PubMed 16842347