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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1MCS4
- Backbone size w/o insert (bp) 4950
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBacterial luciferase
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Alt nameluxCDABE
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SpeciesPhotorhabdus luminescens
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Insert Size (bp)6954
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAKlux1 was a gift from Attila Karsi (Addgene plasmid # 14073 ; http://n2t.net/addgene:14073 ; RRID:Addgene_14073) -
For your References section:
Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring. Karsi A, Menanteau-Ledouble S, Lawrence ML. FEMS Microbiol Lett. 2006 Jul. 260(2):216-23. 10.1111/j.1574-6968.2006.00310.x PubMed 16842347