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Addgene

pET16b-Rnl2(1-249)K227Q
(Plasmid #14072)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16b
  • Backbone manufacturer
    Novagn
  • Backbone size w/o insert (bp) 5711
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rnl2(1-249)
  • Species
    Phage T4
  • Insert Size (bp)
    750
  • Mutation
    changed Lys227 to Glutamine Gly112 is actually Cys112 compared to NCBI
  • GenBank ID
    NP_049790
  • Tag / Fusion Protein
    • His (10x) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Stewart Shuman, Memorial Sloan-Kettering Institute
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16b-Rnl2(1-249)K227Q was a gift from Thomas Tuschl (Addgene plasmid # 14072 ; http://n2t.net/addgene:14072 ; RRID:Addgene_14072)
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