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Addgene

pBS-p85alpha
(Plasmid #1407)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1407 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 2958
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    contains 5' and 3' UTR
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PI3K regulatory subunit p85alpha
  • Alt name
    p85 alpha
  • Alt name
    Pik3r1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3500
  • Entrez Gene
    Pik3r1 (a.k.a. PI3K, p50alpha, p55alpha, p85alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-p85alpha was a gift from Lewis Cantley (Addgene plasmid # 1407 ; http://n2t.net/addgene:1407 ; RRID:Addgene_1407)
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