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Addgene

pJ2048.2
(Plasmid #140687)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCOLA
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3-color stripe (different sgRNAs)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmid pJ1996_v2 is needed for the circuit encoded on this plasmid to operate as designed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ2048.2 was a gift from Yolanda Schaerli (Addgene plasmid # 140687 ; http://n2t.net/addgene:140687 ; RRID:Addgene_140687)

  • For your References section:

    Multistable and dynamic CRISPRi-based synthetic circuits. Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y. Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. 10.1038/s41467-020-16574-1 PubMed 32488086
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