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Addgene

pCINTBeta1
(Plasmid #14067)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2869
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chicken Beta 1 Integrin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    661
  • Mutation
    PstI-BglI fragment was blunt end cloned and EcoRI linkers added and inserted in the EcoRI site.
  • GenBank ID
    M14049
  • Entrez Gene
    ITGB1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINTBeta1 was a gift from Richard Hynes (Addgene plasmid # 14067 ; http://n2t.net/addgene:14067 ; RRID:Addgene_14067)

  • For your References section:

    Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin. Tamkun JW, DeSimone DW, Fonda D, Patel RS, Buck C, Horwitz AF, Hynes RO. Cell. 1986 Jul 18. 46(2):271-82. 10.1016/0092-8674(86)90744-0 PubMed 3487386
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