pJ2077.2
(Plasmid
#140666)
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PurposeController plasmid for TS circuit and cL and cR controls
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameController plasmid for TS circuit and cL and cR controls
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank file with original annotations can be downloaded from the "Resource Information" section.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJ2077.2 was a gift from Yolanda Schaerli (Addgene plasmid # 140666 ; http://n2t.net/addgene:140666 ; RRID:Addgene_140666) -
For your References section:
Multistable and dynamic CRISPRi-based synthetic circuits. Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y. Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. 10.1038/s41467-020-16574-1 PubMed 32488086