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Addgene

pJ2018
(Plasmid #140665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9, LuxR & Csy4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank file with original annotations can be downloaded from the "Resource Information" section.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ2018 was a gift from Yolanda Schaerli (Addgene plasmid # 140665 ; http://n2t.net/addgene:140665 ; RRID:Addgene_140665)

  • For your References section:

    Multistable and dynamic CRISPRi-based synthetic circuits. Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y. Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. 10.1038/s41467-020-16574-1 PubMed 32488086
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