7iBi89 (rat fibronectin)
(Plasmid
#14065)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAGH.Sv
- Backbone size w/o insert (bp) 7700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRat Fibronectin 7iBi89
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Alt nameFibronectin 1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)3115
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MutationInsert was cut out of pGEM3 with SalI and SacI and blunt end cloned into BamHI sites.
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GenBank IDL20801
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Entrez GeneFn1 (a.k.a. FIBNEC, fn-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
7iBi89 (rat fibronectin) was a gift from Richard Hynes (Addgene plasmid # 14065 ; http://n2t.net/addgene:14065 ; RRID:Addgene_14065) -
For your References section:
Elements regulating an alternatively spliced exon of the rat fibronectin gene. Huh GS, Hynes RO. Mol Cell Biol. 1993 Sep . 13(9):5301-14. PubMed 8355683
Map uploaded by the depositor.
