pcFNE3B (chick fibronectin E3B)
(Plasmid
#14064)
-
Depositing Lab
-
Publication
-
Sequence Information
-
Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
-
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 14064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGem-3
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2867
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameChicken Fibronectin EIIIB
-
Alt nameE3B
-
SpeciesG. gallus (chicken)
-
Insert Size (bp)227
-
GenBank IDL20801
-
Entrez GeneFN1 (a.k.a. FN)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcFNE3B (chick fibronectin E3B) was a gift from Richard Hynes (Addgene plasmid # 14064 ; http://n2t.net/addgene:14064 ; RRID:Addgene_14064) -
For your References section:
Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells. Norton PA, Hynes RO. Mol Cell Biol. 1987 Dec . 7(12):4297-307. PubMed 2830487
Map uploaded by the depositor.