pBWB162
(Plasmid
#140634)
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PurposepBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAV1k
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsCan be grown at 30 or 37 C. 30 C used for referenced study in ADP1.
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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Insert Size (bp)711
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byIchiro Matsumura (Addgene #30501)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBWB162 was a gift from Keith Tyo (Addgene plasmid # 140634 ; http://n2t.net/addgene:140634 ; RRID:Addgene_140634) -
For your References section:
Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1. Biggs BW, Bedore SR, Arvay E, Huang S, Subramanian H, McIntyre EA, Duscent-Maitland CV, Neidle EL, Tyo KEJ. Nucleic Acids Res. 2020 Apr 4. pii: 5815822. doi: 10.1093/nar/gkaa167. 10.1093/nar/gkaa167 PubMed 32246719