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Wei Lab Human Whole Genome sgRNA iBAR Library
(Pooled Library #140633)

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  • Purpose

    This human genome-wide CRISPR knockout library incorporates four 6-basepair internal barcodes (iBARs) in each sgRNA.

    Efficient and accurate screening at high MOI is possible by counting both gRNAs and their iBAR sequences.

  • Vector Backbone

    pLenti-sgRNA(lib)-puro (Plasmid #119976) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 140633 Human Whole Genome sgRNA iBAR Library† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with any wild-type spCas9 expressing plasmid such as lentiCas9-Blast (#52962) or otherwise used with cell lines already expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    19,210
  • gRNAs
    58,630
  • Controls
    1,000 non-targeting sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Whole Genome sgRNA iBAR Library was a gift from Wensheng Wei (Addgene #140633)

  • For your References section:

    Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Zhu S, Cao Z, Liu Z, He Y, Wang Y, Yuan P, Li W, Tian F, Bao Y, Wei W. Genome Biol. 2019 Jan 24;20(1):20. doi: 10.1186/s13059-019-1628-0. PubMed 30678704
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