pcFN-C (chick fibronectin)
(Plasmid
#14063)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGem-3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2867
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChicken Fibronectin
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SpeciesG. gallus (chicken)
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Insert Size (bp)166
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MutationDraI - EcoRI fragment inserted in HincII-EcoRI sites
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GenBank IDNM_001198712.1
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Entrez GeneFN1 (a.k.a. FN)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HincII (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcFN-C (chick fibronectin) was a gift from Richard Hynes (Addgene plasmid # 14063 ; http://n2t.net/addgene:14063 ; RRID:Addgene_14063) -
For your References section:
Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells. Norton PA, Hynes RO. Mol Cell Biol. 1987 Dec . 7(12):4297-307. PubMed 2830487