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Addgene

UAST-LbCas12a
(Plasmid #140621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTattB
  • Backbone manufacturer
    Johannes Bischof and Konrad Basler
  • Backbone size w/o insert (bp) 8500
  • Total vector size (bp) 12300
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LbCas12a
  • Alt name
    LbCpf1
  • Insert Size (bp)
    3800
  • Promoter UAST

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAST-LbCas12a was a gift from Michael Boutros (Addgene plasmid # 140621 ; http://n2t.net/addgene:140621 ; RRID:Addgene_140621)

  • For your References section:

    Multiplexed conditional genome editing with Cas12a in Drosophila. Port F, Starostecka M, Boutros M. Proc Natl Acad Sci U S A. 2020 Aug 25. pii: 2004655117. doi: 10.1073/pnas.2004655117. 10.1073/pnas.2004655117 PubMed 32843348
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