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Addgene

pcFNE3A (chick fibronectin E3A)
(Plasmid #14062)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14062 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGem-3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2867
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chicken Fibronectin EIIIA
  • Alt name
    E3A
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    158
  • Mutation
    Blunted BstE2-AvaII fragment was inserted into blunted BamHI-HincII sites
  • GenBank ID
    L20801
  • Entrez Gene
    FN1 (a.k.a. FN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HincII (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcFNE3A (chick fibronectin E3A) was a gift from Richard Hynes (Addgene plasmid # 14062 ; http://n2t.net/addgene:14062 ; RRID:Addgene_14062)

  • For your References section:

    Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells. Norton PA, Hynes RO. Mol Cell Biol. 1987 Dec . 7(12):4297-307. PubMed 2830487
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