pAIDFA-CMV-NmScarlet-mAID
(Plasmid
#140616)
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PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-c1
- Backbone size w/o insert (bp) 5836
- Total vector size (bp) 7628
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1 mutated F74A
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Speciesoriza sativa
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Insert Size (bp)1725
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bymScalet sequence comes from pmScarlet-C1(addgene: #85042)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAIDFA-CMV-NmScarlet-mAID was a gift from Tatsuo Fukagawa (Addgene plasmid # 140616 ; http://n2t.net/addgene:140616 ; RRID:Addgene_140616) -
For your References section:
A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T. Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. 10.1093/nar/gkaa748 PubMed 32941625
