pEIIIB
(Plasmid
#14060)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2869
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRat Fibronectin EIIIB
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SpeciesR. norvegicus (rat)
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Insert Size (bp)255
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MutationEcoRI linkers were attached to HindIII - HinfI fragment.
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GenBank IDL20801
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Entrez GeneFn1 (a.k.a. FIBNEC, fn-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEIIIB was a gift from Richard Hynes (Addgene plasmid # 14060 ; http://n2t.net/addgene:14060 ; RRID:Addgene_14060) -
For your References section:
Cell-type-specific fibronectin subunits generated by alternative splicing. Paul JI, Schwarzbauer JE, Tamkun JW, Hynes RO. J Biol Chem. 1986 Sep 15. 261(26):12258-65. PubMed 3528152
Map uploaded by the depositor.