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pFastBac1-bullfrog saxiphilin
(Plasmid #140595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Gibco Thermo Fisher
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    saxiphilin bullfrog
  • Species
    Rana catesbeiana
  • Mutation
    Deletion of M20 - please see depositor comment
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • GFP-His10 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer polyhedrin forward
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms the M20 deletion does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1-bullfrog saxiphilin was a gift from Dan Minor (Addgene plasmid # 140595 ; http://n2t.net/addgene:140595 ; RRID:Addgene_140595)

  • For your References section:

    Structure of the saxiphilin:saxitoxin (STX) complex reveals a convergent molecular recognition strategy for paralytic toxins. Yen TJ, Lolicato M, Thomas-Tran R, Du Bois J, Minor DL Jr. Sci Adv. 2019 Jun 19;5(6):eaax2650. doi: 10.1126/sciadv.aax2650. eCollection 2019 Jun. 10.1126/sciadv.aax2650 PubMed 31223657
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