pFastBac1-bullfrog saxiphilin
(Plasmid #140595)

• Purpose: protein expression
• Depositing Lab: Dan Minor
• Publication: Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi: 10.1126/sciadv.aax2650. eCollection 2019 Jun.

Backbone

• Vector backbone: pFastBac1
• Backbone manufacturer: Gibco Thermo Fisher
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: saxiphilin bullfrog
• Species: Rana catesbeiana
• Mutation: Deletion of M20 - please see depositor comment
• Promoter: polyhedrin promoter
• Tag / Fusion Protein:
  • GFP-His10 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: polyhedrin forward
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms the M20 deletion does not affect function.