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YCplac33-NAT_MCART1K91A
(Plasmid #140592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac33C
  • Vector type
    Yeast Expression
  • Selectable markers
    NAT (Nourseothricin)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SLC25A51 with 5' and 3' UTR of scNDT1
  • Alt name
    MCART1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    codon-optimized for expression in S. cerevisiae; K91A (lysine 91 mutated to alanine
  • Entrez Gene
    SLC25A51 (a.k.a. CG7943, MCART1)
  • Promoter NDT1 promoter and 3'UTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 rev caggaaacagctatgac
  • 3′ sequencing primer M13 fwd actggccgtcgttttac
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YCplac33-NAT_MCART1K91A was a gift from David Sabatini (Addgene plasmid # 140592 ; http://n2t.net/addgene:140592 ; RRID:Addgene_140592)

  • For your References section:

    MCART1/SLC25A51 is required for mitochondrial NAD transport. Kory N, Uit de Bos J, van der Rijt S, Jankovic N, Gura M, Arp N, Pena IA, Prakash G, Chan SH, Kunchok T, Lewis CA, Sabatini DM. Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. Print 2020 Oct. 10.1126/sciadv.abe5310 PubMed 33087354
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