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Addgene

pEIIIA
(Plasmid #14059)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2869
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rat Fibronectin EIIIA
  • Alt name
    ED
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    213
  • Mutation
    EcoRI linkers added to TaqI to HinfI fragment
  • GenBank ID
    U82612
  • Entrez Gene
    Fn1 (a.k.a. FIBNEC, fn-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEIIIA was a gift from Richard Hynes (Addgene plasmid # 14059 ; http://n2t.net/addgene:14059 ; RRID:Addgene_14059)

  • For your References section:

    Cell-type-specific fibronectin subunits generated by alternative splicing. Paul JI, Schwarzbauer JE, Tamkun JW, Hynes RO. J Biol Chem. 1986 Sep 15. 261(26):12258-65. PubMed 3528152
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