CaV2.1/pcDNA3.1 ~ Human
(Plasmid
#140575)
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PurposeExpression in mammallian cells or oocytes
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaV2.1 Human
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SpeciesH. sapiens (human)
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Entrez GeneCACNA1A (a.k.a. APCA, BI, CACNL1A4, CAV2.1, DEE42, EA2, EIEE42, FHM, HPCA, MHP, MHP1, SCA6)
- Promoter CMV and T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV forward
- 3′ sequencing primer bgh reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaV2.1/pcDNA3.1 ~ Human was a gift from Dan Minor (Addgene plasmid # 140575 ; http://n2t.net/addgene:140575 ; RRID:Addgene_140575) -
For your References section:
A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation. Abderemane-Ali F, Findeisen F, Rossen ND, Minor DL Jr. Neuron. 2019 Mar 20;101(6):1134-1149.e3. doi: 10.1016/j.neuron.2019.01.011. Epub 2019 Feb 4. 10.1016/j.neuron.2019.01.011 PubMed 30733149
