pCS2-mCherry-MKLP1
(Plasmid #140562)

• Purpose: Expresses mCherry tagged MKLP1
• Depositing Lab: Heidi Hehnly
• Publication: Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi: 10.1038/s41467-020-15002-8.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4091
• Total vector size (bp): 8436
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MKLP1
• Alt name: mitotic kinesin-like protein
• Species: H. sapiens (human)
• Insert Size (bp): 2880
• Entrez Gene: KIF23 (a.k.a. CDA3, CDAIII, CDAN3, CDAN3A, CHO1, KNSL5, MKLP-1, MKLP1)
• Promoter: CMV IE94
• Tag / Fusion Protein:
  • 2xmCherry (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 26742 and the MKLP1 gene was a gift from the Doxsey lab at the University of Massachusetts Medical School.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry