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Addgene

p-rlf-1
(Plasmid #14056)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2869
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rat Fibronectin
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    500
  • GenBank ID
    L00191
  • Entrez Gene
    Fn1 (a.k.a. FIBNEC, fn-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-rlf-1 was a gift from Richard Hynes (Addgene plasmid # 14056 ; http://n2t.net/addgene:14056 ; RRID:Addgene_14056)

  • For your References section:

    Three different fibronectin mRNAs arise by alternative splicing within the coding region. Schwarzbauer JE, Tamkun JW, Lemischka IR, Hynes RO. Cell. 1983 Dec . 35(2 Pt 1):421-31. 10.1016/0092-8674(83)90175-7 PubMed 6317187
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