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Addgene

pCS2-H2B-Dendra
(Plasmid #140559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4091
  • Total vector size (bp) 5152
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    H2B
  • Alt name
    Histone 2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    381
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • Dendra (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid 18982

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-H2B-Dendra was a gift from Heidi Hehnly (Addgene plasmid # 140559 ; http://n2t.net/addgene:140559 ; RRID:Addgene_140559)

  • For your References section:

    Cytokinetic bridge triggers de novo lumen formation in vivo. Rathbun LI, Colicino EG, Manikas J, O'Connell J, Krishnan N, Reilly NS, Coyne S, Erdemci-Tandogan G, Garrastegui A, Freshour J, Santra P, Manning ML, Amack JD, Hehnly H. Nat Commun. 2020 Mar 9;11(1):1269. doi: 10.1038/s41467-020-15002-8. 10.1038/s41467-020-15002-8 PubMed 32152267
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