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Addgene

pBabe-Dual
(Plasmid #14055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14055 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6 and H1 promoters
  • Insert Size (bp)
    412
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TCATATGCTTACCGTAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Dual was a gift from Martin Dorf (Addgene plasmid # 14055 ; http://n2t.net/addgene:14055 ; RRID:Addgene_14055)

  • For your References section:

    RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling. Li S, Wang L, Berman MA, Zhang Y, Dorf ME. Mol Cell. 2006 Nov 17. 24(4):497-509. 10.1016/j.molcel.2006.10.015 PubMed 17188031
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