pBV-Luc/Frag-F
(Plasmid #14053)

• Depositing Lab: Joan Massague
• Publication: Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):992-9.

Backbone

• Vector backbone: pBV-luciferase
• Backbone size w/o insert (bp): 4900
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-myc promoter (-109/-3)
• Alt name: c-myc promoter
• Species: H. sapiens (human)
• Insert Size (bp): 106
• Mutation: -109/-3 (relative to the P2 transcription initiation site) of the c-myc promoter.
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

-109/-3 (relative to the P2 transcription initiation site) of the c-myc promoter were amplified by PCR and subcloned into the KpnI/EcoRI site of pBV-Luc, a low basal activity luciferase reporter plasmid.