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Addgene

CS2 Flag-Smad3
(Plasmid #14052)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4100
  • Vector type
    xenopus/mammalian/avian/zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CS2 Flag-Smad3 was a gift from Joan Massague (Addgene plasmid # 14052 ; http://n2t.net/addgene:14052 ; RRID:Addgene_14052)

  • For your References section:

    A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Kretzschmar M, Doody J, Timokhina I, Massague J. Genes Dev. 1999 Apr 1. 13(7):804-16. 10.1101/gad.13.7.804 PubMed 10197981
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