pCMV5 HA-TBRII (delta Cyt)
(Plasmid #14051)

• Depositing Lab: Joan Massague
• Publication: Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 100(14):8430-5.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGF beta rec II (delta cyt)
• Alt name: TGF beta receptor, type II
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Mutation: Dominant negative mutant. A stop codon was introduced just downstream of the transmembrane domain, rendering it incapable of phosphorylating and activating the type I receptor.
• GenBank ID: NM_003242
• Entrez Gene: TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
• Tag / Fusion Protein:
  • HA (PYDVPDYA) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • Diagnostic digest
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry