pCMV5B ActR-IB
(Plasmid
#14050)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV5B
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameActR-IB
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Alt nameActivin receptor, type I B
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank IDU14722
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Entrez GeneACVR1B (a.k.a. ACTRIB, ACVRLK4, ALK4, SKR2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCMV5B is the same as pCMV5 except that EcoRI and BglII sites are removed from the polylinker. See author's map for more information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5B ActR-IB was a gift from Joan Massague (Addgene plasmid # 14050 ; http://n2t.net/addgene:14050 ; RRID:Addgene_14050) -
For your References section:
Activation of signalling by the activin receptor complex. Attisano L, Wrana JL, Montalvo E, Massague J. Mol Cell Biol. 1996 Mar . 16(3):1066-73. PubMed 8622651
Map uploaded by the depositor.
