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Addgene

pCMV5 human p27
(Plasmid #14049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p27
  • Alt name
    p27 Kip1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Entrez Gene
    CDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 human p27 was a gift from Joan Massague (Addgene plasmid # 14049 ; http://n2t.net/addgene:14049 ; RRID:Addgene_14049)

  • For your References section:

    Cell-cycle inhibition by independent CDK and PCNA binding domains in p21Cip1. Luo Y, Hurwitz J, Massague J. Nature. 1995 May 11. 375(6527):159-61. 10.1038/375159a0 PubMed 7753174
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