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Addgene

pCMV5 Flag TGIF
(Plasmid #14047)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TGIF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    TGIF1 (a.k.a. HPE4, TGIF)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See article and author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 Flag TGIF was a gift from Joan Massague (Addgene plasmid # 14047 ; http://n2t.net/addgene:14047 ; RRID:Addgene_14047)

  • For your References section:

    Multiple modes of repression by the Smad transcriptional corepressor TGIF. Wotton D, Lo RS, Swaby LA, Massague J. J Biol Chem. 1999 Dec 24. 274(52):37105-10. 10.1074/jbc.274.52.37105 PubMed 10601270
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