iSKetSnFR2
(Plasmid #140467)

• Purpose: Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
• Depositing Lab: Henry Lester
• Publication: Bera et al Front Cell Neurosci. 2019 Nov 12;13:499. doi: 10.3389/fncel.2019.00499. eCollection 2019.

Backbone

• Vector backbone: pHHM.X513
• Backbone manufacturer: Caltech
• Backbone size w/o insert (bp): 2761
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21(DE3) for bacterial expression and grow at 30 degrees C.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iSKetSnFR2
• Species: bacterial
• Insert Size (bp): 1683
• Promoter: T7
• Tags / Fusion Proteins:
  • His Tag, HA Tag (N terminal on insert)
  • Myc tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry