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Addgene

iSKetSnFR2
(Plasmid #140467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHHM.X513
  • Backbone manufacturer
    Caltech
  • Backbone size w/o insert (bp) 2761
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for bacterial expression and grow at 30 degrees C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iSKetSnFR2
  • Species
    bacterial
  • Insert Size (bp)
    1683
  • Promoter T7
  • Tags / Fusion Proteins
    • His Tag, HA Tag (N terminal on insert)
    • Myc tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iSKetSnFR2 was a gift from Henry Lester (Addgene plasmid # 140467 ; http://n2t.net/addgene:140467 ; RRID:Addgene_140467)

  • For your References section:

    Biosensors Show the Pharmacokinetics of S-Ketamine in the Endoplasmic Reticulum. Bera K, Kamajaya A, Shivange AV, Muthusamy AK, Nichols AL, Borden PM, Grant S, Jeon J, Lin E, Bishara I, Chin TM, Cohen BN, Kim CH, Unger EK, Tian L, Marvin JS, Looger LL, Lester HA. Front Cell Neurosci. 2019 Nov 12;13:499. doi: 10.3389/fncel.2019.00499. eCollection 2019. 10.3389/fncel.2019.00499 PubMed 31798415
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