pL59-Nat
(Plasmid
#140465)
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Purpose(Empty Backbone) CRISPR/Cas9 engineering with nourseothricin selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUD004
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Backbone manufacturerJean-Marc Daran (Addgene plasmid # 101165)
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Modifications to backboneMarker swap of Amidase for antibacterial selection with Nourseothricin
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Vector typeBacterial Expression, Yeast Expression, CRISPR
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlasmid yield is 1-5 ug in a miniprep
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL59-Nat was a gift from Gianni Liti (Addgene plasmid # 140465 ; http://n2t.net/addgene:140465 ; RRID:Addgene_140465) -
For your References section:
Intragenic repeat expansion in the cell wall protein gene HPF1 controls yeast chronological aging. Barre B, Hallin J, Yue JX, Persson K, Mikhalev E, Irizar A, Holt S, Thompson D, Molin M, Warringer J, Liti G. Genome Res. 2020 Apr 10. pii: gr.253351.119. doi: 10.1101/gr.253351.119. 10.1101/gr.253351.119 PubMed 32277013
Map uploaded by the depositor.
