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pCEP4_HA-Halo-cmyc-GRM3 C127A
(Plasmid #140458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone size w/o insert (bp) 10169
  • Total vector size (bp) 13721
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GRM3
  • Alt name
    mGluR3
  • Alt name
    metabotropic glutamate receptor 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3546
  • Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
  • Entrez Gene
    GRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
  • Tags / Fusion Proteins
    • Halo (N terminal on insert)
    • c-myc (N terminal on insert)
    • Signal/leader sequence from influenza hemagglutinin (HA) (N terminal on insert) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4_HA-Halo-cmyc-GRM3 C127A was a gift from Erik Procko (Addgene plasmid # 140458 ; http://n2t.net/addgene:140458 ; RRID:Addgene_140458)
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