pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
(Plasmid #140457)

• Purpose: Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)
• Depositing Lab: Erik Procko
• Publication: Mammalian expression plasmids for production of metabotropic glutamate receptor type 3 (unpublished)

Backbone

• Vector backbone: pCEP4
• Backbone size w/o insert (bp): 10169
• Total vector size (bp): 13721
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GRM3
• Alt name: mGluR3
• Alt name: metabotropic glutamate receptor 3
• Species: H. sapiens (human)
• Insert Size (bp): 2928
• Mutation: Cysteine 127 to alanine, Threonine 174 to alanine
• Entrez Gene: GRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
• Tags / Fusion Proteins:
  • c-myc tag (N terminal on insert)
  • VC: Venus (I152L) C-terminal residues 155-238 (C terminal on insert) (C terminal on insert)
  • Signal/leader sequence from HLA class I histocompatibility antigen A-2 alpha chain (HLA) (N terminal on insert) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV_fwd
• 3′ sequencing primer: EBV_rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry