Skip to main content
Addgene

pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N
(Plasmid #140455)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5344
  • Total vector size (bp) 8026
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GRM3
  • Alt name
    mGluR3
  • Alt name
    metabotropic glutamate receptor 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2676
  • Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
  • Entrez Gene
    GRM3 (a.k.a. GLUR3, GPRC1C, MGLUR3, mGlu3)
  • Tags / Fusion Proteins
    • Signal/leader sequence from HLA class I histocompatibility antigen A-2 alpha chain (HLA) (N terminal on insert) (N terminal on insert)
    • c-myc tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV_fwd
  • 3′ sequencing primer EBV_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N was a gift from Erik Procko (Addgene plasmid # 140455 ; http://n2t.net/addgene:140455 ; RRID:Addgene_140455)
Related items: