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Addgene

DP6
(Plasmid #140446)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140446 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CloDF13
  • Backbone size w/o insert (bp) 2992
  • Total vector size (bp) 9639
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Strains containing DP6 must be maintained in rich media supplemented with 25 mM glucose
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dnaQ926 dam seqA emrR ugi CDA1 gIII

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Received from Ahmed Badran, former Liu lab member.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DP6 was a gift from David Liu (Addgene plasmid # 140446 ; http://n2t.net/addgene:140446 ; RRID:Addgene_140446)

  • For your References section:

    Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021
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