DP6
(Plasmid #140446)

• Purpose: Untargetted bacterial drift
• Depositing Lab: David Liu
• Publication: Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.

Backbone

• Vector backbone: CloDF13
• Backbone size w/o insert (bp): 2992
• Total vector size (bp): 9639
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Strains containing DP6 must be maintained in rich media supplemented with 25 mM glucose
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dnaQ926 dam seqA emrR ugi CDA1 gIII

Resource Information

• Supplemental Documents:
  • DP6.gb
• A portion of this plasmid was derived from a plasmid made by: Received from Ahmed Badran, former Liu lab member.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's NGS found a 65bp insertion in the ori region as compared to the Depositor's sequence. The effect of this insertion is unknown.