Skip to main content
Addgene

N8his-GFPenhancer-GGGGS4-LaG16
(Plasmid #140442)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-28b-Nter8his-HRV3C
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFPenhancer-GGGGS4-LaG16
  • Species
    Synthetic
  • Promoter T7 promotor
  • Tag / Fusion Protein
    • 8 histidine tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHΙ (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N8his-GFPenhancer-GGGGS4-LaG16 was a gift from Motoyuki Hattori (Addgene plasmid # 140442 ; http://n2t.net/addgene:140442 ; RRID:Addgene_140442)

  • For your References section:

    Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Zhang Z, Wang Y, Ding Y, Hattori M. Sci Rep. 2020 Apr 10;10(1):6239. doi: 10.1038/s41598-020-62606-7. 10.1038/s41598-020-62606-7 PubMed 32277083
Related items:
Commonly requested with: