TS_pLuxLac
(Plasmid #140426)

• Purpose: Toggle switch circuit responsive to AHL gradient.
• Depositing Lab: Yolanda Schaerli
• Publication: Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361.

Backbone

• Vector backbone: pZE
• Total vector size (bp): 5797
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GFP

Gene/Insert 2

• Gene/Insert name: LacI

Gene/Insert 3

• Gene/Insert name: TetR

Gene/Insert 4

• Gene/Insert name: mCherry

Resource Information

• Supplemental Documents:
  • pKDL071_pLuxLac.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was modified from pKDL071 described in Litcofsky K.D., Afeyan R.B., Krom R.J., Khalil A.S., Collins J.J.; Iterative plug-and-play methodology for constructing and modifying synthetic gene networks. Nat Methods 9, 1077–1080 (2012). https://doi.org/10.1038/nmeth.2205 Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.