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TS_pLuxLac
(Plasmid #140426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZE
  • Total vector size (bp) 5797
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP

Gene/Insert 2

  • Gene/Insert name
    LacI

Gene/Insert 3

  • Gene/Insert name
    TetR

Gene/Insert 4

  • Gene/Insert name
    mCherry

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was modified from pKDL071 described in Litcofsky K.D., Afeyan R.B., Krom R.J., Khalil A.S., Collins J.J.; Iterative plug-and-play methodology for constructing and modifying synthetic gene networks. Nat Methods 9, 1077–1080 (2012). https://doi.org/10.1038/nmeth.2205 Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TS_pLuxLac was a gift from Yolanda Schaerli (Addgene plasmid # 140426 ; http://n2t.net/addgene:140426 ; RRID:Addgene_140426)

  • For your References section:

    Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch. Barbier I, Perez-Carrasco R, Schaerli Y. Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361. 10.15252/msb.20199361 PubMed 32529808
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