AAV.CBA.eGFP.2A.wtTau
(Plasmid #140424)

• Purpose: AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau
• Depositing Lab: Bradley Hyman
• Publication: Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi: 10.1126/sciadv.aaw6404. eCollection 2019 Jun.

Backbone

• Vector backbone: AAV.CBA.WPRE
• Backbone size w/o insert (bp): 6468
• Total vector size (bp): 8570
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP-2A-Tau
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2102
• GenBank ID: NM_005910.5
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
• Promoter: CBA
• Tags / Fusion Proteins:
  • eGFP (N terminal on insert)
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (unknown if destroyed)
• 3′ cloning site: EcoRV (unknown if destroyed)
• 5′ sequencing primer: do not have
• 3′ sequencing primer: do not have

Resource Information

• Supplemental Documents:
  • pAAV-CBA-eGFP-2A-Tau.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please Note: Plasmid contains a 152bp deletion in the backbone that removes the first 84bp of the ITR upstream of the CAG promoter. This deletion is not known to affect plasmid function.