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Addgene

pCMV5 DPC4 (1-514)
(Plasmid #14040)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DPC4 (1-514)
  • Alt name
    DPC4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    R515 was mutated into a stop codon. AGA to TGA. Dominant negative mutant.
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dominant negative mutant of DPC4. See article and author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 DPC4 (1-514) was a gift from Joan Massague (Addgene plasmid # 14040 ; http://n2t.net/addgene:14040 ; RRID:Addgene_14040)

  • For your References section:

    Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways. Lagna G, Hata A, Hemmati-Brivanlou A, Massague J. Nature. 1996 Oct 31. 383(6603):832-6. 10.1038/383832a0 PubMed 8893010
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