pJBL725
(Plasmid
#140395)
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PurposeExpresses SmtB protein under the control of T7-lacO promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28c
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT7-lacO-SmtB-T
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Tag
/ Fusion Protein
- TEV-6XHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJBL725 was a gift from Julius Lucks (Addgene plasmid # 140395 ; http://n2t.net/addgene:140395 ; RRID:Addgene_140395) -
For your References section:
Cell-free biosensors for rapid detection of water contaminants. Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, Lee JW, Nguyen PQ, Pasten PA, Matiasek SJ, Gaillard JF, Giedroc DP, Collins JJ, Lucks JB. Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 10.1038/s41587-020-0571-7. 10.1038/s41587-020-0571-7 PubMed 32632301
