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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDPC4
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SpeciesH. sapiens (human)
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Insert Size (bp)1700
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Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length DPC4 with N-terminal Flag tag. See author's map for more information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5 Flag-DPC4 was a gift from Joan Massague (Addgene plasmid # 14039 ; http://n2t.net/addgene:14039 ; RRID:Addgene_14039) -
For your References section:
Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways. Lagna G, Hata A, Hemmati-Brivanlou A, Massague J. Nature. 1996 Oct 31. 383(6603):832-6. 10.1038/383832a0 PubMed 8893010
Map uploaded by the depositor.
