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Addgene

pJBL719
(Plasmid #140389)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140389 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7-lacO-QacR-T
  • Tag / Fusion Protein
    • 6XHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL719 was a gift from Julius Lucks (Addgene plasmid # 140389 ; http://n2t.net/addgene:140389 ; RRID:Addgene_140389)

  • For your References section:

    Cell-free biosensors for rapid detection of water contaminants. Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, Lee JW, Nguyen PQ, Pasten PA, Matiasek SJ, Gaillard JF, Giedroc DP, Collins JJ, Lucks JB. Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 10.1038/s41587-020-0571-7. 10.1038/s41587-020-0571-7 PubMed 32632301
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